Cytochrome c Peroxidase. 1. Preparation of the Crystalline Enzyme from Baker's Yeast.
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چکیده
منابع مشابه
Cytochrome c Oxidase from Bakers’ Yeast
Purified cytochrome c oxidase from bakers’ yeast can be resolved into six polypeptide bands by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The apparent molecular weights of these components are I, 42,000; II, 34,500; III, 23,000; IV, 14,000; V, 12,500; and VI, 9,500. (Although Component V actually consists of two distinct polypeptide species, it will be regarde...
متن کاملCytochrome c Oxidase from Bakers’ Yeast
Cytochrome aa3 was purified 35to 40-fold from submitochondrial particles of commercial bakers’ yeast. The purification procedure involved solubilization of the enzyme with cholate, fractionation with ammonium sulfate, and chromatography on DEAE-cellulose in the presence of Triton x-100. The purified, active enzyme contained approximately 10 nmoles of heme a per mg of protein and was free of oth...
متن کاملA method for the preparation of heavy-atom derivatives of yeast cytochrome c peroxidase.
This Letter describes a strategy for preparation of heavy-atom derivatives which has succeeded with yeast cytochrome c peroxidase and which may be useful for other crystalline proteins. Transfer of the crystals from methylpentanediol solutions, in which they were grown, to solutions of polyethylene glycol (34, 6000) maintains the crystal structure so that crystals tolerate heavy-atom concentrat...
متن کاملCytochrome c oxidase from bakers' yeast. II. Site of translation of the protein components.
Purified cytochrome c oxidase from bakers’ yeast can be resolved into six polypeptide bands by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The apparent molecular weights of these components are I, 42,000; II, 34,500; III, 23,000; IV, 14,000; V, 12,500; and VI, 9,500. (Although Component V actually consists of two distinct polypeptide species, it will be regarde...
متن کاملDistribution of cytochrome c peroxidase activity in wild-type and petite cells of bakers' yeast grown aerobically and anaerobically.
Studies of mitochondrial biogenesis in yeast have been hampered by a lack of suitable membrane markers in anaerobically grown cells subsequently grown in air. Cytochrome c peroxidase activity and subcellular location was studied to determine whether it would be a useful marker for an analysis of mitochondrial formation. Cytochemical tests revealed enzyme reaction product on all mitochondrial me...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1967
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.21-0175